Regulatory T-Cell Panel, FOXP3
Also known as: TREGSFOXP3
Use
Regulatory T cells (Tregs) are crucial in suppressing the immune response, primarily through the transcription factor FOXP3. This test is useful for evaluating patients for IPEX syndrome, which is characterized by immune dysregulation. It's also applicable in assessing graft-vs-host disease post hematopoietic stem cell transplantation, and in the context of certain malignancies or autoimmune diseases. Monitoring Tregs provides insight into the mechanism of disease and helps evaluate treatment efficacy.
Special Instructions
Not provided.
Limitations
Severe FOXP3 compromise is identified by low or absent Tregs, which is characteristic of IPEX syndrome. However, certain FOXP3 mutations may inhibit function but still allow detection of the protein by immunologic methods. Thus, absent Tregs by flow cytometry are sufficient, but not necessary, for diagnosis. Pediatric reference ranges have not been established.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
CD4+CD25+FoxP3+CD127-
Cell Type
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
1 mL
Container
Lavender (K2EDTA) or Pink (K2EDTA)
Storage Instructions
Refrigerated
Causes for Rejection
Clotted or hemolyzed specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant
