Special Stain, Luxol Fast Blue
Also known as: LFB SS
Use
This test is a histochemical special stain (Luxol Fast Blue) used in anatomic pathology to visualize myelinated fibers by staining them blue, useful in evaluating neurological tissues and structures. It is a stain-and-return (technical) service only, without interpretive reporting. The test supports visualization of key histologic elements for diagnostic evaluation.
Special Instructions
Anatomic Pathology Test Request Form (ARUP form #32960) is recommended. Submit specimen electronically or via the ARUP form if electronic ordering is unavailable. Use tissue transport kit (ARUP supply #47808). Protect slides or blocks from heat, and ship specimens at room temperature or refrigerated—cooled containers recommended during summer. Frozen specimens are unacceptable. Specimens should not be depleted or nonrepresentative.
Limitations
Limitations include that only tissue or cells prepared as paraffin-embedded blocks or positively charged slides are acceptable; frozen specimens are unacceptable. Depleted specimens or those with nonrepresentative tissue types will be rejected. The test is technical only and does not include interpretive data or clinical report.
New York Approved
Methodology
Other
Biomarkers
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Min: 1 slide
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin preferred) and paraffin embed specimen; cells must be prepared into a cellblock. Transport tissue block or 2 unstained (3- to 5‑micron thick) positively charged slides.
Storage Instructions
Store/transport at room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Nonrepresentative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |