Synaptophysin by Immunohistochemistry
Also known as: SYNAPT IHC
Use
This immunohistochemical stain assesses synaptophysin expression—a marker for neuroendocrine and neuronal differentiation—on formalin-fixed, paraffin‑embedded tissue or cell block preparations. It serves as a technical, stain‑and‑return service used to aid identification and classification of neuronal or neuroendocrine tumors in the histopathologic evaluation context.
Special Instructions
Order via electronic request when capable; otherwise use ARUP Immunohistochemistry Stain Form #32978 with an ARUP client number. Use ARUP’s Immunohistochemistry Stain Offerings brochure for ordering guidance. This test is New York state approved. It is performed as a technical (stain and return) service only.
Limitations
Specimens must be representative tissue or cells; depleted or non‑representative specimens are unacceptable. Slides must be positively charged, 3‑5 microns thick; do not oven bake precut slides. The test provides the stain only; no diagnostic interpretation is included—pathologist interpretation must be ordered separately. Frozen or improperly fixed specimens are unacceptable. Protect paraffin blocks or slides from excessive heat.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 unstained (3- to 5‑micron thick) slides (minimum 2 slides) or a tissue block
Container
Tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or unstained, positively charged slides in ARUP transport kit.
Storage Instructions
Transport at room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens; frozen or improperly fixed specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |