Trofile (DNA) Co-Receptor Tropism
Also known as: TROFI DNA
Use
The test is intended for use in patients with an undetectable viral load to determine the tropism of the HIV-1 virus, specifically whether the virus uses CCR5 or CXCR4 co-receptors, which is crucial for patients being considered for CCR5 antagonist therapy. This determines viral tropism using a pseudovirion-based assay with HIV-1 envelope sequences tested in a cell-based viral infectivity assay.
Special Instructions
Not provided.
Limitations
The test should not be used as the sole criterion for patient management. It uses patient-derived cell-associated viral DNA and may not accurately reflect all viral populations within a patient. Results near the limit of detection may be variable upon subsequent testing.
New York Approved
Methodology
PCR-based
Biomarkers
HIV-1 Co-receptor Tropism
Other
LOINC Codes
- 51969-4 - Gene analysis narr rpt Doc
- 50790-5 - HIV1 RNA tropism Islt
- 80690-1 - HIV1 proviral DNA tropism Bld MC
- 62364-5 - Test performance info Spec
Result Turnaround Time
29-36 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
4 mL
Container
Lavender (K2 or K3EDTA)
Collection Instructions
Freeze immediately and transport in the original collection tube.
Storage Instructions
CRITICAL FROZEN.
Causes for Rejection
Thawed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | Unacceptable |
| Frozen | 2 weeks |
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