Voriconazole, Quantitation by LC-MS/MS
Also known as: VORICON AF
Use
Voriconazole is an azole antifungal drug indicated to treat invasive aspergillosis, candidiasis, scedosporiosis, and fusariosis infections. The therapeutic range is determined based on serum, predose (trough) draw collection at steady-state concentration, which is crucial for optimizing drug therapy and monitoring patient adherence. The pharmacokinetics of voriconazole are influenced by drug-drug interactions, particularly when coadministered with drugs metabolized by cytochrome P450 2C9, 2C19, and 3A4 enzymes, which underscores the importance of this test in therapeutic drug monitoring.
Special Instructions
Specimens should be collected to represent trough levels just before or within 15 minutes of the next dose, or peak levels at specified times post administration. The specimen should be separated from cells as soon as possible, or at least within 2 hours of collection, and transferred to the appropriate transport tube.
Limitations
This is a laboratory-developed test and has not been cleared or approved by the US Food and Drug Administration. As such, it is intended solely for clinical purposes performed in a CLIA certified laboratory. Analytical performance may vary, and physicians should interpret results considering the entire clinical context of the patient. Adverse effects of voriconazole may include nausea, vomiting, tachycardia, and elevated serum liver enzymes.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 38370-3
Result Turnaround Time
1-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.6 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate from cells ASAP or within 2 hours of collection. Transfer to the transport tube.
Patient Preparation
Specimens collected just before or within 15 minutes of the next dose represent the TROUGH levels. Specimens obtained within specific times post administration represent the PEAK level. Collect pre-dose (trough) at steady state concentration.
Storage Instructions
Frozen
Causes for Rejection
Whole blood, gel separator tubes, Light Blue (citrate), or Yellow (SPS or ACD solution) tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 48 hours |
| Frozen | 6 months |