Anti-dsDNA (Double-stranded) Antibodies
Also known as: Anti-N-DNA, Antinative DNA
Use
Specific assay for confirming the diagnosis of systemic lupus erythematosus (SLE)
Special Instructions
This test may exhibit interference when the sample is collected from a person consuming a supplement with a high dose of biotin (vitamin B7 or B8, vitamin H, or coenzyme R). Patients should stop biotin consumption at least 72 hours prior to sample collection. It is advisable to inquire about biotin supplementation when considering this test.
Limitations
Low antibody levels may be found in other connective tissue diseases, making it less predictive for severe nephritis. The presence of these antibodies is not necessary or sufficient for diagnosing SLE, and they may also be seen in other conditions. The test should not be used in isolation but rather as part of a comprehensive diagnostic approach for SLE. Levels of anti-DNA antibodies can help evaluate response to therapy but should be considered a guide rather than a strict treatment directive. Certain medications like procainamide and hydralazine can induce anti-DNA antibodies and antihistone antibodies.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 5130-0
- 5130-0
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube
Patient Preparation
Patients should stop biotin consumption at least 72 hours prior to sample collection.
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; icterus; lipemia; bacterial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |