Antibody Screen
Also known as: Indirect Antiglobulin Test, Indirect Coombs'
Use
Detect atypical IgG antibodies prior to transfusion or during pregnancy. The technique is designed specifically to detect IgG antibodies, though on occasion, some IgM antibodies may also be detected. Antibodies detected by the antibody screen will be subsequently identified, and a titer performed if the antibody identified is considered to be clinically significant during pregnancy.
Special Instructions
If antibodies are detected in the screening process, additional tests, including antibody identification and titer, will be automatically performed at an additional charge to further evaluate the nature and significance of the antibodies detected. This is crucial when preparing for a transfusion or during pregnancies to ensure compatibility and safety.
Limitations
The test does not detect all antibodies, specifically those of the IgM type or antibodies to antigens that occur infrequently. In rare cases, extremely low-titer antibodies may be detected but are too weak to identify. This test is not recommended for specimens known to test positive for red cell antibodies. Additionally, it should not be ordered for the husband or partner of a prenatal patient, as antibodies in their serum or plasma are irrelevant during pregnancy.
Methodology
Immunoassay (Other)
Biomarkers
LOINC Codes
- 890-4
- 890-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
1 mL
Container
Lavender-top (EDTA) tube
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.
Causes for Rejection
Gross hemolysis; bacterial or other contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 2°C to 8°C for up to 72 hours |