Cortisol, Five Specimens
Use
The cortisol test, utilizing five specimens, is employed to evaluate adrenal function and conditions associated with excess or deficient levels of cortisol. It is valuable in diagnosing disorders like Cushing's syndrome or Addison's disease by measuring the hormone levels at different times of the day to understand the circadian rhythm of cortisol secretion.
Special Instructions
Patients scheduled for this test should discontinue biotin supplements, including vitamin B7, at least 72 hours before sample collection as biotin can interfere with the assay. Collect sequential samples as directed.
Limitations
This test may show interference if the patient has consumed biotin supplements. The test is sensitive to sample handling and timing, emphasizing the importance of proper timing and sample collection procedures. Additionally, variations may occur if the usual testing schedule is disrupted, requiring additional confirmatory tests.
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 43215-3
- 21223-3
- 12557-5
- 12558-3
- 12559-1
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Gel-barrier tube (preferred) or red-top tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube.
Patient Preparation
Discontinue biotin supplements at least 72 hours prior to sample collection.
Storage Instructions
Room temperature