Cortisol, Six Specimens
Use
The Cortisol, Six Specimens test is used to assess adrenal gland function by measuring cortisol levels in the serum. This sequential sampling over the course of the day helps in diagnosing conditions related to abnormally high or low cortisol levels, such as Addison's disease or Cushing's syndrome. It provides a detailed diurnal curve, which is essential for understanding the body's cortisol secretion pattern.
Special Instructions
Please refer to the directions for Sequential Sampling. It is important to ask patients about biotin supplementation, as this may interfere with the test results. Patients should cease biotin intake at least 72 hours before sample collection.
Limitations
The test may exhibit interference due to biotin consumption, which can cause inaccurate results. It is essential to ensure the patient has discontinued biotin supplements at least three days prior to sample collection. The volume required (minimum of 0.7 mL) does not allow for repeat testing, thus proper collection and handling are crucial.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 43215-3
- 21223-3
- 12557-5
- 12558-3
- 12559-1
- 12560-9
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Gel-barrier tube (preferred) or red-top tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube.
Patient Preparation
Patients should stop biotin consumption at least 72 hours prior to the collection of a sample.
Storage Instructions
Room temperature