HBV Baseline Evaluation, HCV Direct-acting Antivirals
Also known as: Hepatitis
Use
Risk assessment and management of HBV infection status in patients prior to initiation of HCV treatment with direct-acting antiviral agents.
Special Instructions
If reflex testing is performed, additional charges/CPT codes may apply. It is important to inform patients to refrain from consuming biotin (vitamin B7 or vitamin H) at least 72 hours prior to sample collection, as high doses of biotin can interfere with test results.
Limitations
The HBV quantitative real-time PCR assay used in this evaluation has a quantitative detection range of 10 to 1,000,000,000 IU/mL. This limitation may affect the precise quantification at very low or high levels beyond the specified range. Additionally, biotin interference is a potential limitation if patients do not follow the specified guidelines to avoid biotin supplementation before test collection.
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 77190-7
- 5196-1
- 22322-2
- 13952-7
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7.5 mL
Minimum Volume
2.9 mL
Container
Gel-barrier tube or red-top tube
Collection Instructions
Two samples must be submitted. If tube other than a gel-barrier tube is used, transfer separated serum to a plastic transport tube. Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred).
Patient Preparation
Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Storage Instructions
Room temperature (preferred) up to 14 days. Refrigerated up to 14 days. Frozen up to 14 days. For HBV DNA: Frozen (preferred) up to 12 weeks. Refrigerated up to 6 days. Room temperature up to 24 hours.
Causes for Rejection
Heat-inactivated specimens; cord blood; cadaveric specimens; or body fluid other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |