Hepatitis B Virus (HBV) Prenatal Screen With Reflex to HBV DNA
Use
Screening of pregnant women for hepatitis B virus (HBV) infection to identify neonates who are at risk of acquiring hepatitis B during the perinatal period. A positive HBsAg test will reflex to HBV quantitative DNA.
Special Instructions
If the reflex test is performed, additional charges/CPT codes may apply. Patients should be screened for biotin supplement consumption, as it can interfere with the test results. They should be advised to discontinue biotin intake at least 72 hours before sample collection.
Limitations
Patients who are negative for HBsAg may still have acute type B viral hepatitis due to antigen levels below the detection limit of the assay or lack of antigen reactivity. Strong clinical suspicion of viral hepatitis should lead to a comprehensive test battery that includes detection of antibodies hepatitis B core antigen (anti-HBc) and HBV DNA.
Methodology
Immunoassay (ICMA)
Biomarkers
LOINC Codes
- 5196-1
- 5196-1
- 7905-3
- 42595-9
- 48398-2
- 19147-8
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
6 mL
Minimum Volume
2.2 mL
Container
Gel-barrier tube or serum transfer tube
Collection Instructions
Centrifuge sample within 24 hours of collection. If a tube other than a gel-barrier tube is used, or if the sample will be shipped frozen, transfer separated serum to a screw-cap polypropylene transport tube. Do not freeze gel-barrier tube; pour off serum first.
Causes for Rejection
Non-EDTA plasma specimen; PST gel-barrier tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | HBsAg: Room temperature up to 14 days. |
| Refrigerated | HBsAg: Refrigerated up to 14 days. |
| Frozen | HBsAg: Frozen up to 7 days. HBV DNA: Frozen (preferred) up to 12 weeks. |