Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay
Also known as: HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay, HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay
Use
Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.
Special Instructions
Indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number or unique identifier for serial monitoring. Note that separate frozen specimens should be submitted for each test requested to avoid delays in turnaround time when requesting multiple tests on frozen samples.
Limitations
The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. It requires an HCV genotype of either 1a or 1b. Incorrect specimen handling - such as using heparinized plasma, or nonfrozen, hemolyzed, or improperly transferred specimens - can result in rejection of the sample and potentially affect accuracy or lead to no result.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 11011-4
- 11011-4
- 38180-6
- 80563-0
- 72862-6
- 73655-3
- 49549-9
Result Turnaround Time
11-17 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
4 mL
Minimum Volume
2 mL
Container
Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in 'pop-top' or 'snap-cap' tube; whole blood