Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A
Also known as: HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A, HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A
Use
Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.
Special Instructions
Please ensure that the patient's HCV genotype (either 1a or 1b) is indicated on the test request form. This information is necessary for the reflex testing to the HCV GenoSure® NS3/4A.
Limitations
The quantitative range for this HCV real-time PCR assay is 15 IU/mL to 100,000,000 IU/mL. Accurate results cannot be guaranteed outside this range. The test requires the presence of HCV genotype either 1a or 1b for accurate processing and subsequent reflex testing to HCV GenoSure® NS3/4A. Specimens not meeting the volume requirements or improperly processed (e.g., not centrifuged within six hours, non-frozen specimens) may lead to test rejection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 75888-8
- 11011-4
- 38180-6
- 32286-7
- 77202-0
Result Turnaround Time
11-17 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
4 mL
Minimum Volume
2 mL
Container
Lavender-top (EDTA) tube, Plasma Preparation Tube (PPT™), gel-barrier tube
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in 'pop-top' or 'snap-cap' tube; whole blood