Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A
Also known as: HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A, HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A
Use
Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.
Special Instructions
Not provided.
Limitations
The quantitative range for this HCV real-time PCR assay is 15 IU/mL to 100,000,000 IU/mL. Accurate results cannot be guaranteed outside this range. The test requires the presence of HCV genotype either 1a or 1b for accurate processing and subsequent reflex testing to HCV GenoSure® NS3/4A. Specimens not meeting the volume requirements or improperly processed (e.g., not centrifuged within six hours, non-frozen specimens) may lead to test rejection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 75888-8 - HCV RNA MU Set Patient
- 11011-4 - HCV RNA SerPl NAA+probe-aCnc
- 38180-6 - HCV RNA SerPl NAA+probe-Log IU
- 32286-7 - HCV Gentyp SerPl NAA+probe
- 77202-0 - Laboratory comment Report
Result Turnaround Time
11-17 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
4 mL
Minimum Volume
2 mL
Container
Lavender-top (EDTA) tube, Plasma Preparation Tube (PPT™), gel-barrier tube
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in 'pop-top' or 'snap-cap' tube; whole blood
Other tests from different labs that may be relevant