Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) NS5B Drug Resistance Assay
Use
Quantification of HCV with reflex to HCV NS5B drug resistance assay (at additional charge) if ≥1000 IU/mL.
Special Instructions
Not provided.
Limitations
The real-time PCR assay has a quantitative range from 15 IU/mL to 100,000,000 IU/mL. It requires the patient's HCV genotype 1a or 1b for reflex testing to the NS5B drug resistance assay. Non-adherence to specimen collection and handling procedures, including hemolysis, use of heparinized plasma, and non-frozen specimens, can lead to invalid results or rejection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 50023-1 - HCV RNA Pnl SerPl NAA+probe
- 11011-4 - HCV RNA SerPl NAA+probe-aCnc
- 38180-6 - HCV RNA SerPl NAA+probe-Log IU
- 72862-6 - HCV resis panell Islt Genotyp
- 73655-3 - HCV NS5 Mut Det Islt Genotyp
- 49549-9 - Ref lab test method
Result Turnaround Time
11-17 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
4 mL
Minimum Volume
2 mL
Container
Ship in plasma preparation tube (PPT™) or screw-capped polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood
Other tests from different labs that may be relevant