Histoplasma capsulatum Antibodies, Complement Fixation
Use
The Histoplasma capsulatum Antibodies test, using complement fixation methodology, is designed to detect the presence of antibodies against Histoplasma capsulatum in serum. This test aids in diagnosing infections caused by Histoplasma capsulatum, a dimorphic fungus that can lead to histoplasmosis, particularly in individuals exposed to environments with bird or bat droppings. Early detection of antibodies can assist in timely medical evaluation and treatment to prevent severe pulmonary or disseminated disease, especially in immunocompromised patients.
Special Instructions
The test requires careful handling of serum, with specific container requirements such as a red-top tube or gel-barrier tube. The minimum serum volume is 0.6 mL, and the refrigerating of specimens is crucial to maintain stability for up to 14 days. Accurate sample volume is essential since volumes less than 0.6 mL may not allow for repeat testing, impacting result verification.
Limitations
The test has limitations including potential cross-reactivity with antibodies from other fungal infections, which may lead to false positives. The complement fixation method may not detect early antibodies at very low concentrations or in cases where the immune response is insufficient. Furthermore, the results must be interpreted within the context of clinical and epidemiological evidence as serology cannot distinguish between latent and active infection.
Methodology
Other
Biomarkers
LOINC Codes
- 90227-0
- 20573-2
- 20574-0
Result Turnaround Time
2-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.6 mL
Container
Red-top tube or gel-barrier tube
Storage Instructions
Refrigerate.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |
| Frozen | 14 days |