Human Antimouse Antibodies
Also known as: HAMA
Use
This test is intended for the detection and semi-quantitation of human antibodies to mouse IgG (HAMA).
Special Instructions
Not provided.
Limitations
The presence of Rheumatoid Factor (RF) and/or heterophilic antibodies in the sample may interfere with the assay and could result in false-positive values. Lipemic serum samples may also interfere with the assay, resulting in false-positive values. HAMA has been shown to interfere with some two-site immunometric assays utilizing murine monoclonal antibodies, potentially causing both false positive and false-negative results.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 14975-7
- 14975-7
Result Turnaround Time
6-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.4 mL
Minimum Volume
0.2 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Once the serum has been separated from the clot, it may be stored at 2-8ºC for up to 24 hours. If the serum specimen cannot be tested within one day of collection, the specimen should be stored at -70ºC until tested. Serum samples are stable at -70ºC for at least three months. Freeze and thaw only once.
Storage Instructions
Freeze.
Causes for Rejection
Nonserum specimen; gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Not established |
| Refrigerated | 24 hours |
| Frozen | 3 months |