Human Immunodeficiency Virus 1 & 2 (HIV-1/HIV-2), Qualitative, RNA
Use
This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.
Special Instructions
Not provided.
Limitations
Due to the potential cross-reactivity between some NAT assays and certain lentiviral vector-based CAR-T cell therapies, clinicians should exercise caution when ordering and interpreting HIV testing in patients who have received CAR-T cell therapy. Some lentiviral CAR-T vectors contain a target region of the test, which can lead to false positive HIV-1 results.
Methodology
PCR-based (RT-PCR)
Biomarkers
HIV-1 RNA
MicroorganismHIV-2 RNA
Microorganism
LOINC Codes
- 96557-4 - HIV 1 + 2 RNA Pnl SerPl NAA+probe
- 25835-0 - HIV1 RNA SerPl Ql NAA+probe
- 69353-1 - HIV 2 RNA SerPl Ql NAA+probe
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
650 µL
Container
Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube
Collection Instructions
Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.
Storage Instructions
Refrigerated or frozen
Causes for Rejection
Specimen grossly hemolyzed; specimen received at room temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 6 weeks |
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