Human Immunodeficiency Virus 1 (HIV-1) GenoSure PRIme® Plus Trofile®
Use
Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors (GenoSure PRIme®) and detect HIV-1 coreceptor tropism (Trofile®); determine eligibility for CCR5 antagonist therapy, such as maraviroc (Selzentry®)
Special Instructions
Ensure collection in lavender-top (EDTA) or plasma preparation tubes (PPT™). Centrifuge within six hours and transfer plasma to screw-cap polypropylene transport tubes. Avoid using heparinized tubes. Ship specimens frozen to avoid delays and accommodate turnaround times.
Limitations
Trofile® is recommended for patients with documented HIV-1 infection with viral load ≥1000 copies/mL. GenoSure PRIme® may not succeed if HIV viral load is <500 copies/mL plasma. Failure to obtain sufficient virus for genotype results will necessitate HIV-1 RNA quantification, billed separately. This test’s performance characteristics were determined by Labcorp; it lacks FDA clearance or approval.
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 80689-3
- 51969-4
- 62364-5
- 50790-5
- 57182-8
- 77202-0
- 49549-9
- 75608-0
Result Turnaround Time
24-28 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
20 mL
Minimum Volume
Not provided
Container
Lavender-top (EDTA) or plasma preparation tube (PPT™)
Collection Instructions
Collect in four lavender-top (EDTA) or four PPT™ tubes. Centrifuge within six hours. Transfer plasma to screw-cap polypropylene transport tubes. Ship frozen.
Storage Instructions
Freeze for shipment or long-term storage.
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; received in 'pop-top' or 'snap-cap' tubes