Luteinizing Hormone (LH)
Also known as: Interstitial Cell-stimulating Hormone
Use
The primary clinical use of LH measurement is in evaluating the normalcy of hypothalamic-pituitary-gonadal axis. Measurement of serum gonadotropin levels will allow for distinguishing between primary gonadal failure and deficient gonadal stimulation. LH measurement may also be of clinical importance because growth hormone and LH are frequently the first hormones to be affected by pituitary disease. The serum analysis of LH has also been found to be very useful in the diagnosis and treatment of infertility in women.
Special Instructions
This test may exhibit interference if the sample is collected from a person consuming a high dose of biotin (vitamin B7/B8, vitamin H, coenzyme R). Patients should be asked about biotin supplementation and advised to stop intake at least 72 hours before sample collection.
Limitations
Secretion of both LH and FSH is pulsatile due to the intermittent release of gonadotropin-releasing hormone (GnRH). In females, FSH and LH levels vary over the menstrual cycle, peaking at ovulation. Interpretation of a single determination may be difficult. It is suggested to obtain samples at 15-30 minute intervals and pool equal serum volumes to minimize pulsatile secretion effects. Erroneous findings may occur due to monoclonal mouse antibodies or interference from high antibody titers against streptavidin and ruthenium. Additives minimize these effects.
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 10501-5
- 10501-5
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Red-top tube, gel-barrier tube or green-top (lithium heparin) tube. Do not use oxalate, EDTA or citrate plasma.
Collection Instructions
If a red-top tube or plasma is used, transfer separated serum or plasma to a plastic transport tube.
Patient Preparation
Patients should stop biotin supplements at least 72 hours prior to collection.
Storage Instructions
Room temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |