Lyme Antibodies, Modified 2-Tier Testing Profile, Serum/Plasma

Also known as: B. burgdorferi, Borrelia burgdorferi, Lyme disease, MTTT, Tickborne Disease

Clinical Use

Use

This panel utilizes FDA-cleared assays following the modified 2-tier testing (MTTT) algorithm to aid in the diagnosis of Lyme disease in individuals with clinical signs and symptoms consistent with Lyme disease. Lyme disease should be considered based on the presence of typical signs and symptoms of infection in patients with a history of possible exposure to infected ticks.

Special Instructions

Not provided.

Limitations

Screening of the general population should not be performed. The positive predictive value depends on the likelihood of Lyme disease being present. Testing should only be performed on patients with clinical symptoms of Lyme disease or when exposure is suspected. In general, IgM tests should be disregarded if the patient's symptoms have lasted more than 30 days. If the patient has been sick longer than 30 days, only IgG results should be interpreted. Potential assay interference due to circulating antibodies in patients with Human Ehrlichiosis (HE) and Tick Borne Relapsing Fever (TBRF), Babesiosis, Parvovirus, and EBV infections has been found. Interpret results from these patients with caution.

Test Details

FDA Approved

Methodology

Immunoassay (CLIA)

Biomarkers

Lyme IgG

Antibody

Other

Other • Binary (Detected / Not Detected)

Lyme IgM

Antibody

Other

Other • Binary (Detected / Not Detected)

Lyme Total Antibody

Antibody

Other

Other • Binary (Detected / Not Detected)

LOINC Codes

Order Codes

98205-8 - B burgdor IgG + IgM + tot Pnl Ser IA

Result Codes

83081-0 - B burgdor IgG+IgM Ser Ql IA
16480-6 - B burgdor IgG SerPl Ql IA
40612-4 - B burgdor IgM SerPl Ql IA
101358-0 - B burgdor Ab Ser IA.MTTT-Imp

Result Turnaround Time

1-2 days

Specimen

Serum

Volume

2.0 mL

Minimum Volume

0.75 mL

Container

Gel-barrier tube, serum transfer tube

Collection Instructions

If tube other than a gel-barrier is used, transfer separated serum or plasma to a labeled plastic transport tube. Do not freeze gel-barrier tube (pour off serum first).