Lyme Antibodies, Modified 2-Tier Testing Profile, Serum/Plasma
Also known as: B. burgdorferi, Borrelia burgdorferi, Lyme disease, MTTT, Tickborne Disease
Use
This panel utilizes FDA-cleared assays following the modified 2-tier testing (MTTT) algorithm to aid in the diagnosis of Lyme disease in individuals with clinical signs and symptoms consistent with Lyme disease. Lyme disease should be considered based on the presence of typical signs and symptoms of infection in patients with a history of possible exposure to infected ticks.
Special Instructions
Specimens are tested using an immunoassay measuring total antibodies to specific borrelial proteins; if reactive, the specimens reflex to separate IgG and IgM immunoassays targeting additional Borrelia burgdorferi proteins.
Limitations
Screening of the general population should not be performed. The positive predictive value depends on the likelihood of Lyme disease being present. Testing should only be performed on patients with clinical symptoms of Lyme disease or when exposure is suspected. In general, IgM tests should be disregarded if the patient's symptoms have lasted more than 30 days. If the patient has been sick longer than 30 days, only IgG results should be interpreted. Potential assay interference due to circulating antibodies in patients with Human Ehrlichiosis (HE) and Tick Borne Relapsing Fever (TBRF), Babesiosis, Parvovirus, and EBV infections has been found. Interpret results from these patients with caution.
FDA Approved
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 98205-8
- 83081-0
- 16480-6
- 40612-4
- 101358-0
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2.0 mL
Minimum Volume
0.75 mL
Container
Gel-barrier tube, serum transfer tube
Collection Instructions
If tube other than a gel-barrier is used, transfer separated serum or plasma to a labeled plastic transport tube. Do not freeze gel-barrier tube (pour off serum first).
Storage Instructions
Room temperature
Causes for Rejection
Gross hemolysis; gross lipemia; samples containing particulate matter or exhibiting obvious microbial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |