Phospholipase A2 Receptor Autoantibodies, IgG
Also known as: Antibodies to the M-type Phospholipase A2 Receptor
Use
The anti-PLA2R ELISA (IgG) test kit is intended for the qualitative or semiquantitative determination of IgG class autoantibodies against phospholipase A2 receptor (PLA2R) in human serum.1 It is used as an aid in the diagnosis of primary membranous glomerulonephritis (pMGN), in conjunction with other laboratory and clinical findings.
Special Instructions
The test is conducted using an enzyme-linked immunosorbent assay (ELISA) methodology, and it is important for the sample to be suitably collected, generally in a red-top or gel-barrier tube. If a red-top tube is used, the separated serum should be transferred to a plastic transport tube.
Limitations
The test is not a standalone test but an adjunct to other clinical information. A diagnosis of membranous nephropathy (MN) should not be made on a single test result. Absence of circulating PLA2R autoantibodies does not rule out a diagnosis of PLA2R-related membranous nephropathy. The clinical symptoms, results on physical examination, and laboratory tests should always be taken into account when considering the diagnosis of primary versus secondary MN. The assay has not been demonstrated to be effective for monitoring the stage of disease or its response to treatment. Borderline samples should be considered as potentially positive and retested.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 73737-9
- 73737-9
Result Turnaround Time
2-8 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube.
Storage Instructions
Room temperature
Causes for Rejection
Freeze/thaw cycles Stable x3
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |