Pregnenolone, Pre- and Post-ACTH Stimulation

Also known as: Pregnenolone (Two Specimens)

Clinical Use

Use

This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.

Special Instructions

Not provided.

Limitations

The test may not accurately reflect adrenal function in the presence of interfering substances or conditions affecting pregnenolone levels. Repeat testing is not possible with the minimum volume. Accuracy of results is dependent on proper collection and handling of specimens. Results outside the expected ranges should be correlated with clinical findings and other diagnostic procedures.

Test Details

Methodology

Mass Spectrometry

Biomarkers

LOINC Codes

Result Codes

2837-3 - Preg SerPl-mCnc
46100-4 - Preg 1h p 250 ug ACTH SerPl-mCnc

Result Turnaround Time

7-9 days

Specimen

Serum

Volume

1 mL (each specimen)

Minimum Volume

0.4 mL

Container

Red-top tube, gel-barrier tube

Collection Instructions

Draw two samples: one baseline and one stimulated 60 minutes later. If not using a gel-barrier, transfer separated serum or plasma to a plastic transport tube. Label each tube with the patient's name, collection time, and date. Submit specimens simultaneously on the same test request form.