Bordetella pertussis toxin (PT) Antibodies (IgG, IgA), MAID
Also known as: Whooping Cough
Use
Antibody levels above the reference range are highly suggestive of recent infection or vaccination. PT IgG is the best serologic test for identifying recent pertussis infection in individuals of all ages, with PT IgA clinically useful in children less than 10 years old to help distinguish recent infection from recent vaccination. Measurement of PT antibodies is preferred for patients with persistent cough lasting more than 2–3 weeks when PCR sensitivity declines. PT antibodies are reported in IU/mL; values above reference range suggest recent infection or recent vaccination. PT is specific for B pertussis, whereas other antigens lack specificity. Reference ranges vary by age for both IgG and IgA. These assays cannot assess protective immunity. The purpose is to aid diagnosis in patients with persistent cough or recent exposure. (Derived from various Quest sources.)
Special Instructions
Not provided.
Limitations
These assays cannot be used to assess protective immunity to B pertussis, because the antibody levels that correlate with protection are not well defined. Beyond 2–3 weeks of cough onset, PCR sensitivity declines and serology is preferred. IgA adds value in distinguishing infection from vaccination in children under 10. (Based on Quest clinical FAQ.)
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Container
Red Top or Gold SST tube
Causes for Rejection
Gross hemolysis; lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient: 7 days |
| Refrigerated | Refrigerated: 14 days |
| Frozen | Frozen: 30 days |