Follicular Lymphoma BCL‑2/JH t(14;18) Real‑Time PCR (Cell‑Based)
Also known as: bcl-2/JH t(14;18)
Use
This test qualitatively detects the IGH/BCL2 t(14;18) translocation, involving the immunoglobulin heavy chain (JH region) and BCL2 gene, commonly associated with follicular lymphoma. The translocation is one of the most frequent cytogenetic abnormalities in follicular lymphoma and can also be detected in other lymphoid malignancies. The t(14;18) fusion aids in differential diagnosis and disease monitoring where neoplastic burden is sufficient.
Special Instructions
Not provided.
Limitations
This assay detects rearrangements involving the major (MBR), minor (MCR), or intermediate (ICR) breakpoint cluster regions, covering approximately 70–80% of t(14;18) events. It does not detect BCL2 rearrangements involving non‑IGH partners. The assay limit of detection is approximately 1 in 10,000 translocation‑bearing cells; translocations may not be detected in samples with neoplastic burden below this threshold. Furthermore, IGH‑BCL2 fusions may be detected in blood or lymph nodes of individuals without neoplasia using sensitive PCR methods.
Methodology
PCR-based (PCR)
Biomarkers
BCL2, IGH
Gene
Result Turnaround Time
3-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
Not provided
Container
EDTA (lavender top) tube
Collection Instructions
Collect blood in a lavender top tube; refrigerate and send within 48 hours.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | Within 48 hours |
| Frozen | Frozen on dry ice |
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