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HIV-1 RNA, Quantitative PCR with Reflex to Genotype — Quest Diagnostics | Casandra.ai

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Casandra Test Code QD28455Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 34471CPT Codes 87536, 87900, 87901

HIV-1 RNA, Quantitative PCR with Reflex to Genotype

Also known as: HIV-1 RNA, HIV Quant, HIV Genotyping

Clinical Use

Use

Quantifies HIV-1 RNA (viral load) in plasma of individuals with known HIV‑1 infection. If the viral load meets or exceeds a specified threshold (commonly ≥400 copies/mL in Quest’s algorithm), the test reflexes to genotypic HIV‑1 resistance testing to identify drug resistance mutations. Useful for monitoring disease progression, assessing response to antiretroviral therapy, and guiding regimen changes in cases of therapeutic failure.

Special Instructions

Not provided.

Limitations

Not provided.

Test Details

Methodology

PCR-based (PCR)

Biomarkers

2 components

HIV‑1 RNA Quantitative PCR • PCRHIV‑1 Genotype (reflex) • PCR

Result Turnaround Time

1-2 days

Related Documents

For more information, please review the documents below

Lab test overview

Specimen Requirements

Specimen

Plasma

Volume

2 mL (1 mL minimum) in each of two tubes

Minimum Volume

Not provided

Container

EDTA (lavender-top) or PPT (white-top) tubes

Causes for Rejection

Frozen plasma in PPT; serum; whole blood >24 hours old; frozen whole blood; specimens collected in lithium or sodium heparin

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