HIV-1 RNA, Quantitative PCR with Reflex to Genotype
Also known as: HIV-1 RNA, HIV Quant, HIV Genotyping
Use
Quantifies HIV-1 RNA (viral load) in plasma of individuals with known HIV‑1 infection. If the viral load meets or exceeds a specified threshold (commonly ≥400 copies/mL in Quest’s algorithm), the test reflexes to genotypic HIV‑1 resistance testing to identify drug resistance mutations. Useful for monitoring disease progression, assessing response to antiretroviral therapy, and guiding regimen changes in cases of therapeutic failure.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL (1 mL minimum) in each of two tubes
Minimum Volume
Not provided
Container
EDTA (lavender-top) or PPT (white-top) tubes
Causes for Rejection
Frozen plasma in PPT; serum; whole blood >24 hours old; frozen whole blood; specimens collected in lithium or sodium heparin