HIV‑1 RNA, Quantitative, Real‑Time PCR, with Reflex to Integrase Genotype
Also known as: Human Immunodeficiency Virus
Use
This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV‑1 infected patients. It can be used to assess patient prognosis by measuring the baseline HIV‑1 RNA level, or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV‑1 RNA levels during the course of treatment. Additionally, the emergence of integrase drug resistance mutations can be observed and inform therapy adjustments by amplifying and sequencing the HIV‑1 integrase gene, reporting mutations at positions associated with integrase inhibitor drug resistance.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
PCR‑based (RT‑PCR)
Biomarkers
HIV‑1 RNA
Analyte
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
Not provided
Minimum Volume
3 mL plasma collected in an EDTA (lavender‑top) tube
Container
EDTA (lavender‑top) tube
Collection Instructions
Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Samples submitted in primary tube will be rejected.
Causes for Rejection
Gross hemolysis; lipemia; samples received in primary collection tube
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 42 days |
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