PML/RARA t(15;17), Quantitative, Real Time‑PCR
Also known as: Translocation (15;17), PML/RARA, Quantitative RT-PCR, AML(M3), APL
Use
This assay detects the short form (bcr3), long form (bcr1) and the variant exon 6 (bcr2) PML‑RARA transcripts associated with the t(15;17) chromosomal translocation. PML‑RARA transcript levels are expressed as normalized copy number (NCN) of PML‑RARA using ABL1 as internal control. Optimal for diagnosis and monitoring of acute promyelocytic leukemia (APL).
Special Instructions
Only accept extracted RNA when extraction or isolation is performed in an appropriately qualified laboratory such as a CLIA‑certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or CMS. Include date and time of draw and sample type on tube. Insurance authorization is required prior to draw for outpatients; inpatients are acceptable to send without prior authorization.
Limitations
Not provided.
Methodology
PCR-based (RT-PCR)
Biomarkers
Result Turnaround Time
5-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
4 mL
Container
EDTA (lavender‑top) tube
Collection Instructions
Collect 5 mL whole blood in an EDTA (lavender‑top) tube. Whole blood or bone marrow collected in ACD‑B (yellow‑top) tube or sodium heparin (green‑top) tube also acceptable.
Storage Instructions
Room temperature or refrigerated; do not freeze whole blood or bone marrow.
Causes for Rejection
Clotted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |
| Refrigerated | 5 days |
| Frozen | Unacceptable |