ANA Screen, IFA with Reflex to Titer and Pattern / Systemic Sclerosis Panel 1

Casandra

Casandra Test Code QD84110Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 90073CPT Code 86038

ANA Screen, IFA with Reflex to Titer and Pattern / Systemic Sclerosis Panel 1

Also known as: Calcinosis, Esophageal dysmotility, Topoisomerase I Antibody, CENP B Antibody, FANA

Clinical Use

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Use

This test begins with an ANA screen using immunofluorescence assay (IFA) on HEp‑2 cells to detect antinuclear antibodies, a common marker in autoimmune rheumatic diseases; if positive, it reflexes to determine ANA titer and staining pattern, which aids in suggesting specific diagnoses such as systemic sclerosis and other autoimmune conditions ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).

Special Instructions

Reflex testing to ANA titer and pattern is performed automatically when the ANA IFA screen is positive ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).

Limitations

A negative ANA IFA result suggests absence of ANA-associated autoimmune disease but does not rule it out; certain antibodies (e.g., Jo‑1, SSA) may still be present despite negative ANA, especially in myositis or SLE/Sjögren syndrome ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).

Test Details

Methodology

Immunoassay (IHC)

Biomarkers

ANA Titer

Analyte

ANA titer • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

Antinuclear antibodies (ANA)

Analyte

ANA IFA screen • Other • Binary (Detected / Not Detected)

Staining pattern

Analyte

Pattern identification • Other • Categorical (e.g., Positive / Negative / Indeterminate)

Result Turnaround Time

3-4 days

Specimen

Serum

Volume

Not provided

Minimum Volume

Not provided

Container

Gel‑barrier tube (SST, Tiger Top)

Collection Instructions

Collect and label sample according to standard protocols; invert tube gently 5 times immediately after draw; allow blood to clot 30 minutes; centrifuge for 10 minutes.