Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot
Also known as: BORDPAN
Use
The Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot test is used to detect antibodies against Bordetella pertussis, the causative agent of whooping cough. This test aids in the diagnosis of pertussis infection, differentiating between acute and convalescent phases based on IgM and IgG antibody titers. ELISA results help to determine immune status after exposure or vaccination. A positive result in this test may indicate a current or recent infection or immunization against Bordetella pertussis.
Special Instructions
Mark specimens plainly as 'acute' or 'convalescent.' Parallel testing is preferred, and convalescent specimens should be received within 30 days from the receipt of the acute samples. Serum must be separated from cells as soon as possible or within two hours of collection.
Limitations
This test is not approved for use in New York State. The IgM ELISA may yield false-positive results, hence immunoblot reflex is recommended for positive results to confirm diagnosis. Additionally, negative results must be considered in conjunction with clinical presentation as serological responses may be delayed or absent after vaccination or early infection.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 42329-3
- 42330-1
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection. Mark specimens plainly as 'acute' or 'convalescent.'
Causes for Rejection
Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |
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