Hepatitis C Virus Genotype by Sequencing
Also known as: HEPCGENO
Use
The Hepatitis C Virus Genotype by Sequencing test identifies the genotype of Hepatitis C virus present in a patient sample. Genotypes are crucial for determining treatment strategies and predicting outcomes. This test utilizes RT-PCR to amplify a portion of the viral genome's 5' UTR, followed by sequencing. The results are compared to a database to determine the specific HCV genotype, with types 1-6 being the main categories. Due to genomic similarities, it may not differentiate subtype 1a from 1b, and in rare cases, type 6 may be misclassified as type 1.
Special Instructions
Do not order this test prior to molecular confirmation of a positive hepatitis C screen. Serum or plasma must be separated from cells ASAP or within 24 hours post-collection for accurate results. Unacceptable conditions include heparinized specimens.
Limitations
The assay cannot differentiate between HCV subtypes 1a and 1b, nor between rare type 6 and type 1 due to high conservation in the 5' UTR region. A higher resolution genotyping test (ARUP test code 2006898) is available for more precise subtype differentiation. Additionally, the test may fail if viral load is less than log 3.6 or 4000 IU/mL, and it may be affected by PCR inhibitors or viral genetic variation.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 92731-9
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
2 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA), pink (K2EDTA), plasma preparation tube (PPT) or serum separator tube (SST)
Collection Instructions
Separate from cells ASAP or within 24 hours of collection.
Causes for Rejection
Heparinized specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 72 hours |
| Frozen | 4 months |
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