Hepatitis C Virus (HCV) Genotyping With 1a Subtype Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay
Use
Genotyping of the six major HCV types and their most common subtypes. Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Detects resistance-associated variants at positions 28, 30, 31, and 93 in genotype 1a HCV.
Special Instructions
Reflex testing is automatically triggered when genotype 1a is identified. Additional time may be required for reflex testing. Samples should be submitted as separate frozen specimens when multiple frozen tests are requested to avoid delays in turnaround.
Limitations
Genotyping may be unsuccessful when HCV viral load is less than 1000 IU/mL in serum or plasma. The NS5A drug resistance assay is only performed when genotype 1a is detected.
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
12-18 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
2 mL
Container
PPT™ or screw‑capped polypropylene frozen transport tubes
Collection Instructions
Collect in two PPT™ tubes, gel‑barrier tubes, ACD (yellow‑top), or EDTA (lavender‑top) tubes; do not use heparin (green‑top). Centrifuge within six hours, remove serum/plasma, transfer to polypropylene screw‑capped tubes, freeze and ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; use of green‑top (heparin) tube; specimen not frozen on date of collection; PPT™ not centrifuged; specimen received in pop‑top or snap‑cap tube; specimen shipped at room temperature
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