Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A

Casandra

Casandra Test Code LC88618Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 550072CPT Code 87522

Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Hepatitis C Virus (HCV) GenoSure® NS3/4A

Also known as: HCV Real-time PCR With Reflex to HCV GenoSure® NS3/4A, HCV RNA Quantitation With Reflex to HCV GenoSure® NS3/4A

Clinical Use

Order Test

Use

Quantification of HCV with reflex to HCV GenoSure NS3/4A drug resistance (at additional charge) if ≥2000 IU/mL. Detects Q80K polymorphism.

Special Instructions

Please ensure that the patient's HCV genotype (either 1a or 1b) is indicated on the test request form. This information is necessary for the reflex testing to the HCV GenoSure® NS3/4A.

Limitations

The quantitative range for this HCV real-time PCR assay is 15 IU/mL to 100,000,000 IU/mL. Accurate results cannot be guaranteed outside this range. The test requires the presence of HCV genotype either 1a or 1b for accurate processing and subsequent reflex testing to HCV GenoSure® NS3/4A. Specimens not meeting the volume requirements or improperly processed (e.g., not centrifuged within six hours, non-frozen specimens) may lead to test rejection.

Test Details

Methodology

PCR-based (RT-PCR)

Biomarkers

LOINC Codes

Order Codes

75888-8

Result Codes

11011-4
38180-6
32286-7
77202-0

Result Turnaround Time

11-17 days

Specimen

Plasma

Volume

4 mL

Minimum Volume

2 mL

Container

Lavender-top (EDTA) tube, Plasma Preparation Tube (PPT™), gel-barrier tube

Collection Instructions

Collect specimen in two PPT™ tubes, gel-barrier tubes or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes and freeze. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship frozen.