Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

Casandra

Casandra Test Code LC17100Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 550333CPT Code 87522

Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

Also known as: HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay, HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay

Clinical Use

Order Test

Use

Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.

Special Instructions

Indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number or unique identifier for serial monitoring. Note that separate frozen specimens should be submitted for each test requested to avoid delays in turnaround time when requesting multiple tests on frozen samples.

Limitations

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. It requires an HCV genotype of either 1a or 1b. Incorrect specimen handling - such as using heparinized plasma, or nonfrozen, hemolyzed, or improperly transferred specimens - can result in rejection of the sample and potentially affect accuracy or lead to no result.

Test Details

Methodology

PCR-based (qPCR)

Biomarkers

HCV NS5A Drug Resistance

Other

NS5A Resistance Assay • Other • Binary (Detected / Not Detected)

Hepatitis C Virus RNA

Analyte

HCV RNA Quantitation • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

HCV log10 • Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Order Codes

11011-4

Result Codes

11011-4
38180-6
80563-0
72862-6
73655-3
49549-9

Result Turnaround Time

11-17 days

Specimen

Plasma

Volume

4 mL

Minimum Volume

2 mL

Container

Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.

Collection Instructions

Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen.