Use

This panel can be helpful in the diagnosis and management of systemic lupus erythematosus (SLE). The panel simultaneously tests for antinuclear antibodies (ANAs) using an immunofluorescence assay (IFA) on HEp‑2 cells, as well as six specific autoantibodies associated with SLE and three biomarkers associated with disease activity. Reflex testing for ANA titer and pattern is performed for specimens with a positive ANA IFA result. The ANA screen has high sensitivity (97%) but limited specificity (34%) for SLE; anti‑dsDNA and anti‑Sm antibodies are less sensitive but more specific and both are included in SLE classification criteria. Anti‑chromatin antibodies have relatively high sensitivity and specificity. Although SSA, SSB, and RNP antibodies are more associated with Sjögren syndrome or mixed connective tissue disease, they are also frequently present in SLE and may be diagnostically helpful. Results should be interpreted in clinical context including patient history and physical findings.