Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded Antibody Panel by IIF With ELISA
Also known as: PNP PLUS
Use
The Paraneoplastic Pemphigus (Paraneoplastic Autoimmune Multiorgan Syndrome) Expanded Antibody Panel is used to aid in the diagnosis and monitoring of paraneoplastic pemphigus. This condition is a rare paraneoplastic disease associated with lymphoproliferative disorders or malignancies and exhibits clinical features of severe pemphigus with a high mortality rate. The test detects IgG antibodies to multiple epithelial antigens, including envoplakin, which correlate with the extent of mucocutaneous disease in patients with this syndrome. Monitoring the antibody levels can assist in assessing disease expression and activity, particularly in response to therapy.
Special Instructions
This test should be distinguished from antibody testing of cerebral spinal fluid (CSF) for paraneoplastic neurologic syndromes. For comprehensive testing, it can be ordered along with other panels such as the Immunobullous Disease Antibody Panel, Pemphigus Antibody Panel, IgG and IgA, and the Basement Membrane Zone Antibody Panel to assess for specific disease-associated antibodies.
Limitations
The test is performed in a CLIA-certified laboratory and not FDA cleared or approved, as FDA clearance is not required for clinical purposes. The sensitivity of indirect immunofluorescence can vary, and while ELISA is generally more sensitive for detecting antibodies, it may not detect low levels. Negative results do not rule out paraneoplastic/malignancy-associated disease, and clinical correlation is essential with histopathological examination and other epithelial antibodies in serum. Various tests might have differing sensitivities and specificities for detecting antibodies associated with PNP/PAMS.
New York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 11502-2
Result Turnaround Time
7-14 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
0.5 mL
Container
Plain red or serum separator tube (SST)
Collection Instructions
Transfer 2 mL serum to an ARUP standard transport tube.
Storage Instructions
Refrigerated
Causes for Rejection
Hemolyzed or lipemic specimens. Plasma.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 week |
| Refrigerated | 2 weeks |
| Frozen | Indefinitely |
Other tests from different labs that may be relevant