Protein Electrophoresis, 24-Hour Urine
Also known as: Electrophoresis, Protein, 24-Hour Urine, Globulins, 24-Hour Urine
Use
Evaluate myeloma, macroglobulinemia of Waldenström, lymphoma, amyloidosis; differentiate between normal renal function, glomerular proteinuria, and tubular proteinuria. Increased glomerular permeability leads to higher concentrations of large proteins in the glomerular filtrate. Diminished tubular reabsorptive capacity results in a marked increase in urinary excretion of low molecular weight problems.
Special Instructions
Record the total 24-hour urine volume on the test request form. It’s advised that a serum protein electrophoresis be reviewed if not previously studied, as urine patterns often mirror serum patterns in glomerular proteinuria. Urine contamination with blood can cause the same pattern.
Limitations
The test might not detect pathologic light chains due to insufficient sensitivity, and should be followed with immunoelectrophoretic or immunofixation studies on concentrated urine. The optimal specimen for free monoclonal light chain detection (Bence Jones protein) is a 24-hour urine collection.
Methodology
Other
Biomarkers
LOINC Codes
- 81231-3
- 2888-6
- 2889-4
- 13986-5
- 13984-0
- 13987-3
- 13988-1
- 13989-9
- 42484-6
- 42482-0
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
20-mL aliquot
Minimum Volume
6-mL aliquot (Note: This volume does not allow for repeat testing.)
Container
12-mL plastic transport tube (Labcorp No. 23597) without preservative
Collection Instructions
Instruct patient to void at 8 a.m. and discard the specimen. Then collect all urine for the next 24 hours, including the 8 a.m. void the next morning. Label container with the patient's name, date and time the collection started and ended. Measure total volume. Mix well.
Storage Instructions
Refrigerated (preferred) or room temperature
Causes for Rejection
Preservative added
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |
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