Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot
Also known as: BORDPAN2
Use
This test is recommended for assessing late-stage pertussis (>4 weeks) to determine immune response via IgA and IgG antibodies against Bordetella pertussis. It is specifically designed for situations where serology is used to aid diagnosis of pertussis infection in the late stage, as PCR and culture may not be as effective. If elevated levels of IgA or IgG antibodies are detected, it may suggest a current or past exposure to the organism or immunization.
Special Instructions
Not provided.
Limitations
While this test is effective for assessing antibodies in the later stages of potential exposure to Bordetella pertussis, it is not FDA approved and performance characteristics have not been established by them. Notably, bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens are conditions that may lead to sample rejection. Repeat freeze/thaw cycles could negatively impact the integrity of frozen specimens as well.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 42328-5 - B pert IgA Ser IA-aCnc
- 42330-1 - B pert IgG Ser IA-aCnc
- 42328-5 - B pert IgA Ser IA-aCnc
- 42330-1 - B pert IgG Ser IA-aCnc
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection. Transfer to an ARUP Standard Transport Tube.
Storage Instructions
Refrigerated.
Causes for Rejection
Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |
Other tests from different labs that may be relevant