Chimerism, Posttransplant, Sorted Cells (T Cells)
Also known as: STRPOST-T
Use
This test is used to monitor engraftment of donor cells post allogeneic stem cell transplant. It assists in determining the proportion of donor and recipient cells in a posttransplant setting. The analysis is performed to correlate with clinical status and consider the interval between bone marrow transplantation and testing for proper interpretation of results.
Special Instructions
Each posttransplant specimen and test ordered must have a unique ARUP accession number. Donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Information regarding patient's most recent WBC and differential counts, and general cellularity of the patient's bone marrow should be provided. See Cell Isolation Request for Chimerism, Posttransplant, Sorted Cells.
Limitations
Diagnostic errors can occur due to rare sequence variations. The test has a limit of detection for 2 percent of the minor cell population. Clotted or hemolyzed specimens are unacceptable for this test.
New York Approved
Methodology
PCR-based (Fragment Analysis)
Biomarkers
Result Turnaround Time
5-10 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
2 mL
Minimum Volume
2 mL
Container
Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B)
Collection Instructions
Transport 2 mL whole blood. Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s).
Storage Instructions
Refrigerated. Also acceptable: Ambient.
Causes for Rejection
Clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |