Clostridium difficile Cytotoxin Cell Assay
Also known as: CDIFTX
Use
The Clostridium difficile Cytotoxin Cell Assay is used to detect the presence of cytotoxins produced by Clostridium difficile in stool samples. It is not the preferred initial test for the diagnosis; institutional guidelines recommend alternatives like the Clostridium difficile toxin B gene detection by PCR or a glutamate dehydrogenase and/or toxin detection test. This assay is important for confirming the presence of cytotoxin in suspected cases to guide treatment decisions.
Special Instructions
It is important to consult institutional policies and guidelines for selecting the appropriate test for diagnosing Clostridium difficile infection. The specimen should be transferred into an unpreserved stool transport vial and the acceptable storage and transport temperature is refrigerated. Orders should be coordinated using ARUP supply and client services as needed.
Limitations
The assay is sensitive to conditions such as stability during transportation and storage. It is unsuitable for samples preserved in preservatives or submitted on swabs. Stability is limited to specific conditions: ambient for 2 hours, refrigerated for up to 72 hours, frozen at -20°C for 72 hours, and frozen at -70°C for up to 1 week. Optimal results might not be attained if these conditions are not met.
New York Approved
Methodology
Cell-based / Cytometry
Biomarkers
LOINC Codes
- 6362-8
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Stool
Volume
1 mL
Minimum Volume
0.5 mL
Container
Unpreserved stool transport vial (ARUP supply #40910)
Collection Instructions
Transfer stool to an unpreserved stool transport vial using ARUP supply #40910.
Storage Instructions
Refrigerate the specimen for transport.
Causes for Rejection
Specimens in preservatives or submitted on swabs.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 hours |
| Refrigerated | 72 hours |
| Frozen | 72 hours at -20 degrees C; 1 week at -70 degrees C |