Casandra
Casandra Test Code QD54123Version 1 (DRAFT)
Performing Lab
Clostridium difficile Culture with Reflex to Toxin B, Real‑Time PCR, Isolate
Clinical Use
Order TestUse
C. difficile causes 50‑75% of antibiotic‑associated colitis and greater than 90% of antibiotic‑associated pseudomembranous colitis. Pathogenic C. difficile produce two potent toxins, toxin A and toxin B, that cause diarrhea and colitis.
Special Instructions
Not provided.
Limitations
Not provided.
Test Details
Methodology
Culture‑based
Biomarkers
Targets not provided.
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen Requirements
Specimen
Stool
Volume
5 g or 5 mL
Minimum Volume
2 g or 2 mL
Container
sterile screw‑cap container
Collection Instructions
Toxin is heat labile. Freeze as soon as possible. Submit frozen stool in sterile screw‑cap container.
Causes for Rejection
Received room temperature; received in Cary‑Blair transport; specimen other than liquid or semi‑formed stool; stool in preservative or mixed with urine; specimen in wrong transport container
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 48 hours |
| Frozen | 7 days at −70 °C (not established at −20 °C) |
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