E-Cadherin by Immunohistochemistry
Also known as: ECADH IHC
Use
E-Cadherin by Immunohistochemistry (IHC) is used primarily in the evaluation of epithelial cell adhesion and differentiation. The test detects E-cadherin, a protein that is part of the cadherin superfamily, which is involved in calcium-dependent cell-cell adhesion. Loss or reduction of E-cadherin expression is associated with various types of carcinomas, including lobular breast carcinoma and diffuse gastric cancer, making it an important biomarker in the diagnostic assessment of cancer progression and prognosis.
Special Instructions
IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. For additional technical details, contact ARUP Client Services at (800) 522-2787.
Limitations
This test is performed as a stain and return (technical) service only, meaning that ARUP Laboratories does not provide interpretive pathology services for this test. The performance and interpretation of the staining results are the responsibility of the pathologist at the client laboratory. Specimens submitted with non-representative tissue types or depleted specimens may lead to inaccurate or invalid results.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Paraffin block or 5 unstained slides (3- to 5-micron thick sections), positively charged slides in a tissue transport kit
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or slides using a tissue transport kit.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |