Flunitrazepam and Metabolites, Serum or Plasma, Screen with Reflex to Confirmation/Quantitation
Also known as: FLUNITR SP
Use
This test is used to detect the exposure to Flunitrazepam, commonly known by its brand name Rohypnol, and its metabolites in serum or plasma. Detection is performed using qualitative high-performance liquid chromatography-tandem mass spectrometry. It supports clinical toxicology, particularly in cases requiring emergency toxicology assessment for benzodiazepines. The reflex to confirmation and quantitation allows for accurate confirmation and measurement of detected substances when the initial screen is positive.
Special Instructions
Specimen must be separated from cells as soon as possible, ideally within 2 hours of collection. Only plasma or serum collected in plain red, lavender (EDTA), or pink (K2EDTA) tubes is accepted. Avoid separator tubes as they are unacceptable. The test has a reflex confirmation process, and additional charges may apply for the confirmation test.
Limitations
The test is not performed at ARUP Laboratories but by a contracted laboratory, which may result in variations in testing protocols and detection limits. The effectiveness of the test in detecting Flunitrazepam and its metabolites can be influenced by the time elapsed post-ingestion and the dosage. Detection limits are set at 2.0 ng/mL, and results below these levels will not be reported. Cross-reactivity with other substances and the possibility of false positives/negatives should be considered when interpreting results.
New York Approved
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 23864-2
- 32650-4
- 21046-8
Result Turnaround Time
5-13 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
3 mL
Minimum Volume
1.4 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Collect in plain red, lavender (EDTA) or pink (K2EDTA) tube. Separate from cells as soon as possible or within 2 hours of collection.
Storage Instructions
Refrigerate after collection. Specimens may also be frozen.
Causes for Rejection
Separator tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 3 months |