Glial Fibrillary Acidic Protein (GFAP) by Immunohistochemistry
Also known as: GFAP IHC
Use
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein expressed in glial cells such as astrocytes and ependymal cells. Immunohistochemical (IHC) staining for GFAP is useful for identifying glial origin in tissues, particularly helpful in diagnosing glial tumors like astrocytomas and ependymomas.
Special Instructions
Not provided.
Limitations
Specimens must be formalin-fixed (10% neutral buffered formalin) and paraffin-embedded (cells in cell block). Do not oven bake precut slides. Slides/blocks must contain representative tissue and not be depleted. Frozen specimens are unacceptable. Slide immunoreactivity may degrade over time; older, poorly charged, or overheated slides can reduce stain quality.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or 5 unstained positively charged slides (3–5 micron thick sections)
Collection Instructions
Protect paraffin block and/or slides from excessive heat. If sending precut slides, do not oven bake.
Storage Instructions
Ship at room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non-representative tissue type; depleted specimens; frozen specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant