Heroin - Screen with Reflex to Confirmation/Quantitation - Urine
Also known as: HEROIN URN
Use
This test is used to detect the presence of heroin and its metabolites in urine samples. It is primarily used in clinical settings to screen for heroin use and diagnose substance abuse cases. The test involves an initial screening using an enzyme immunoassay. If the screening result is positive, confirmation testing using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is performed to quantify specific heroin metabolites such as morphine and 6-Monoacetylmorphine (6-MAM).
Special Instructions
The test is performed by a non-ARUP laboratory and requires specific specimen preparation and handling conditions. It is important to ensure that urine specimens are collected and stored properly to maintain the stability of heroin metabolites, particularly 6-Monoacetylmorphine, which can degrade if the sample is not frozen.
Limitations
The test may return false-negative results if the urine sample is not properly frozen, as 6-Monoacetylmorphine is unstable at higher temperatures. Analytical limitations exist due to the method sensitivity and specificity for detecting heroin metabolites among other opiates. Reporting limits are in place, and samples with drug concentrations below this threshold may not be detected. Further, the test is not FDA-cleared or approved, which may limit its acceptance in certain clinical protocols.
New York Approved
Methodology
Immunoassay (Enzyme Immunoassay)
Biomarkers
LOINC Codes
- 3547-7
Result Turnaround Time
4-10 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
2 mL
Minimum Volume
1 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Collect random urine.
Causes for Rejection
Thawed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 48 hours |
| Frozen | 3 months |