PM/Scl-100 Antibody, IgG by Immunoblot
Also known as: PM/SCL
Use
The PM/Scl-100 IgG antibody test is primarily used to evaluate the presence of connective tissue diseases, such as polymyositis (PM), dermatomyositis (DM), systemic sclerosis (SSc), and polymyositis/systemic sclerosis overlap syndrome. It is significant when the PM/Scl-100 IgG antibody is present alongside a positive ANA IFA nucleolar pattern. The test helps in differential diagnosis of systemic sclerosis or connective tissue disease with overlapping features of systemic sclerosis and/or myositis.
Special Instructions
Collect serum in a serum separator tube. Transfer 1 mL of serum to an ARUP standard transport tube for processing. Use caution to avoid plasma contamination and hemolysis.
Limitations
This test detects antibodies to PM/Scl-100, but antibodies to other target epitopes in the PM/Scl complex are not captured by this assay. The clinical relevance of detecting PM/Scl-100 IgG antibodies in the absence of a positive ANA IFA nucleolar pattern remains unknown. Interpreting test results should consider the entire clinical picture and not rely solely on this test.
New York Approved
Methodology
Immunoassay (Qualitative Immunoblot)
Biomarkers
LOINC Codes
- 81732-0
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP standard transport tube
Collection Instructions
Collect using a serum separator tube and transfer 1 mL serum.
Causes for Rejection
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 month |