Chromosome Analysis - Prenatal - Amniotic Fluid with AFP
Use
Chromosome Analysis - Prenatal - Amniotic Fluid with AFP is essential for diagnosing fetal chromosome abnormalities, providing clarity for cases with prenatal ultrasound findings indicating issues. It aids in analyzing important aspects such as chromosomal size and structure using G-banding techniques. The test plays a vital role in pregnancy management, particularly in high-risk scenarios and cases of advanced maternal age. Additionally, this analysis also measures alpha fetoprotein (AFP), linked to neural tube defects, enhancing the testing's significance in prenatal diagnostics. Genetic counselors are available for consultations before sample submissions, ensuring proper guidance and coordination.
Special Instructions
For prenatal cases, consultation with our laboratory is required before submitting samples. Genetic counselors are available to assist in coordinating the testing process. It is recommended to follow specific guidelines for specimen collection and shipping to ensure the accuracy of results and proper management of samples. Results should always be interpreted along with ultrasonography findings, as a positive result may not confirm an anomaly.
Limitations
This standard cytogenetic methodology may not detect subtle chromosome rearrangements that are less than 10 Mb or low-level mosaicism. Chromosomal Microarray Analysis may be needed for microdeletions/microduplications. It is essential to note that standard cytogenetic analysis does not rule out other genetic conditions or birth defects that might not be detectable. Fetal cell cultures are kept for seven days, after which they are discarded if no further testing is requested. Positive AFP results must be evaluated in conjunction with ultrasound findings.
Methodology
Chromosomal / Cytogenetics (Karyotype)
Biomarkers
Result Turnaround Time
10 days
Related Documents
Not provided.
Specimen
Amniotic Fluid
Volume
20-30 cc
Minimum Volume
N/A
Container
two sterile 15 ml conical centrifuge tubes
Collection Instructions
Collect 20-30 cc of fluid in two sterile 15 ml conical centrifuge tubes. Discard the first 2 cc of fluid collected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |