Oncology FISH Analysis – 1p/19q Co-deletion

Clinical Use

Use

Oncology FISH Analysis – 1p/19q Co-deletion is a Fluorescence In Situ Hybridization (FISH) test designated to detect a common co-deletion associated with classic oligodendroglioma histology. Oligodendrogliomas typically manifest as central nervous system tumors and often lead to symptoms such as seizures, headaches, and physical weakness. The deletion of the short arm of chromosome 1 and the long arm of chromosome 19 is a genetic signature for oligodendrogliomas, which has been correlated with improved patient survival. Accurate interpretation of results necessitates consideration of pertinent clinical and pathological data and is not intended to serve as a standalone diagnostic tool for oligodendroglioma.

Special Instructions

Specimens for FISH testing must be fixed in 10% neutral buffered formalin for a minimum of 6 hours but not exceeding 48 hours. The volume of formalin used should at least be 10 times greater than the specimen volume. Avoid using decalcification solutions with strong acids as they may compromise specimen integrity. Samples fixed for less than 6 or greater than 48 hours may yield false negatives in testing results.

Limitations

This FISH assay is specifically designed to test for the co-deletion of the 1p and 19q loci. It does not identify any abnormalities beyond these specified loci, limiting its diagnostic scope.

Test Details

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

2 components

Primary Component • FISHFISH Analysis • FISH

Result Turnaround Time

7 days

Specimen

Tumor Tissue (FFPE)

Volume

10 cc

Minimum Volume

10 cc

Container

FFPE Tumor Block

Collection Instructions

Transport Temperature: ambient (18-25°C/64-77°F). Protect from excessive heat. Unacceptable conditions: No tumor in tissue. Frozen specimens, specimens fixed/processed in alternative fixatives.