17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation
Also known as: 17-Hydroxypregnenolone (Two Specimens), Pregnenolone, 17-Hydroxy (Two Specimens)
Use
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Special Instructions
Draw blood for baseline 17-OH pregnenolone before administering cosyntropin. Inject 250 μg of cosyntropin intramuscularly or intravenously, if using IV, dilute in 2-5 mL of sterile saline and inject over two minutes. Draw blood for stimulated 17-OH pregnenolone 60 minutes after injection. Submit specimens simultaneously on the same test request form to avoid delays.
Limitations
Results should be interpreted by qualified personnel familiar with the test, considering patient history and clinical presentation. Accuracy may be affected by recently administered isotopes. Minimum sample volume may not allow for repeat testing. Results may vary based on sample stability and handling.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 6765-2
- 6765-2
- 13604-4
Result Turnaround Time
7-12 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.4 mL
Container
Red-top tube, gel-barrier tube
Collection Instructions
Transfer separated serum to a plastic transport tube. Label each tube with patient's name, collection time, and date.
Storage Instructions
Freeze
Causes for Rejection
Non-EDTA/heparin plasma; recently administered isotopes
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 1 day |
| Frozen | 2 years |