ANA by IFA, Reflex to 11-biomarker Profile, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B, Sm/RNP, Ribosomal P by Multiplex Immunoassay
Also known as: ANA by IFA Reflex
Use
Assay for screening for ANAs in patients suspected of having connective tissue disease. The indirect immunofluorescent test has three elements to consider in the result:
Special Instructions
Not provided.
Limitations
Patients older than 80 years have a 50% incidence of low titer ANA. Certain medications can induce a lupoid condition and elevated ANA titers, which usually decrease once the drug is discontinued. Low antibody levels may occur in diseases other than connective tissue diseases, reducing the diagnostic specificity of the test for such conditions.
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
LOINC Codes
- 42254-3 - ANA Ser Ql IF
- 5130-0 - dsDNA Ab Ser-aCnc
- 29374-6 - ENA RNP Ab Ser-aCnc
- 11090-8 - ENA SM Ab Ser-aCnc
- 27048-8 - ENA SM+RNP Ab Ser-aCnc
- 27416-7 - ENA Scl70 Ab Ser-aCnc
- 17792-3 - ENA SS-A Ab Ser-aCnc
- 17791-5 - ENA SS-B Ab Ser-aCnc
- 51775-5 - Chromatin Ab SerPl-aCnc
- 13636-6 - Ribosomal P Ab Ser-aCnc
- 11565-9 - ENA Jo1 Ab Ser-aCnc
- 53982-5 - Centromere B Ab Ser-aCnc
- 20398-4 - ANA Homogen Titr Ser
- 20399-2 - ANA nucleolar Titr Ser
- 20401-6 - ANA Speckled Titr Ser
- 5077-3 - Centromere Ab Titr Ser IF
- 53003-0 - Mitotic spindle app Ab Titr SerPl IF
- 54006-2 - ANA nuclear membrane pores Titr Ser IF
- 54155-7 - ANA intercell bridge Titr Ser IF
- 53998-1 - ANA nuclear dots Titr Ser IF
- 53006-3 - ENA PCNA Ab Titr Ser IF
- 53993-2 - ANA centrosomal Titr Ser IF
- 8251-1 - Service Cmnt-Imp
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 7 days |
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