ANA Screen, IFA with Reflex to Titer and Pattern / Systemic Sclerosis Panel 1
Also known as: Calcinosis, Esophageal dysmotility, Topoisomerase I Antibody, CENP B Antibody, FANA
Use
This test begins with an ANA screen using immunofluorescence assay (IFA) on HEp‑2 cells to detect antinuclear antibodies, a common marker in autoimmune rheumatic diseases; if positive, it reflexes to determine ANA titer and staining pattern, which aids in suggesting specific diagnoses such as systemic sclerosis and other autoimmune conditions ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).
Special Instructions
Reflex testing to ANA titer and pattern is performed automatically when the ANA IFA screen is positive ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).
Limitations
A negative ANA IFA result suggests absence of ANA-associated autoimmune disease but does not rule it out; certain antibodies (e.g., Jo‑1, SSA) may still be present despite negative ANA, especially in myositis or SLE/Sjögren syndrome ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq177?utm_source=openai)).
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
Not provided
Minimum Volume
Not provided
Container
Gel‑barrier tube (SST, Tiger Top)
Collection Instructions
Collect and label sample according to standard protocols; invert tube gently 5 times immediately after draw; allow blood to clot 30 minutes; centrifuge for 10 minutes.
Storage Instructions
Store serum at 2 °C to 8 °C after collection and ship the same day.
Causes for Rejection
Gross hemolysis; gross lipemia; gross icterus; microbial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 days |
| Refrigerated | 7 days |
| Frozen | 30 days |